Metabonomics studies on plasma and urine of patients with breast cancer using NMR spectroscopy are to establish metabolic markers correlated with breast cancer to guide clinic diagnosis and provide the new ideas and methods. In this study, a total of 45 patients with breast cancer were enrolled and 50 healthy volunteers as controls. Meanwhile, blood and urine samples from the subjects were collected by the researchers for testing. For each sample, the 1H nuclear magnetic resonance (NMR) based metabonomic analysis was performed, the subsection integral of the free attenuation signal was computed, and the obtained data were analyzed by the orthogonal partial least-squares discriminant analysis (OPLS-DA). The results of the study show that compared with the healthy people, leucine, valine, phenylalanine, tyrosine, alanine, histidine, and glycine are significantly decreased (P<0.05); lipid (LDL), beta glucose，lactic acid, choline, inositol and creatine are significantly reduced (P<0.05); glycoprotein, malonic acid, acetoacetic acid, beta hydroxybutyric acid, and acetone are increased significantly (P<0.05) in the plasma of patients with breast cancer. Moreover, isoleucine, valine, glutamic acid, histidine, dimethylamine, hippuric acid, betaine, taurine, succinic acid, citric acid,creatine, and creatinine are decreased (P<0.05) and Lactic acid increased (P<0.05) in urine of patients with breast cancer. Through this study, metabolic markers correlated with breast cancer using changes of metabonomics in plasma and urine have been established.
[1] 陈海彬, 周红光, 俞晓忆, 等. 代谢组学在中药现代化研究中的应用[J]. 中国中西医结合杂志, 2012, 32(6): 831-835． Chen Haibin, Zhou Hongguang, Yu Xiaoyi, et al. The application of metabonomics in modern studies of chinese materia medica[J]. Chinese Journal of Integrative Medicine, 2012, 32(6): 831-835．
[2] 朱嘉, 方祝元. 代谢组学与中医“证”的相关性[J]. 现代中西医结合杂志, 2009, 18(8): 938-941． Zhu Xi, Fang Zhuyuan. The correlation between metabonomics and TCM syndrome[J]. Modern Journal of Integrated Traditional Chinese and Western Medicine, 2009, 18(8): 938-941．
[3] 郑杰. 肿瘤生长的能量代谢特点及临床应用[J]. 中国细胞生物学学报, 2011, 33(10): 1158-1165. Zheng Jie. Energy metabolism and clinical application of tumor growth[J]. Chinese Journal of Cell Biology, 2011, 33(10): 1158-1165.
[4] 陆敬华, 刘世育. 谷氨酸对SW1990 胰腺癌细胞系体外增殖的影响[J]. 实用医学杂志2009, 25(7): 1025-1027. Lu Jinghua, Liu Shiyu. Effects of glutamate on the in vitro proliferation of pancreatic carcinoma cell line SW1990[J]. The Journal of Practical Medicine, 2009, 25(7): 1025-1027.
[5] 袁忠民, 徐利民, 王建刚, 等. 谷氨酸促脑胶质瘤细胞侵袭的机制研究[J]. 岭南现代临床外科, 2009, 9(6): 456-458. Yuan Zhongmin, Xu Limin, Wang Jiangang, et al. Study on the mechanism of invasion evoked by glu tamine in cerebral glioma cells[J]. Lingnan Morden Clinics in Surgery, 2009, 9(6): 456-458.
[6] 查锡良, 周春燕. 生物化学[M]. 7版. 北京: 人民卫生出版社, 2008. Zha Xiliang, Zhou Chunyan. Biochemistry[M]. 7th ed. Beijing: People's Medical Publishing House, 2008.
[7] 王勇, 李中峰, 陈建新, 等. 基于冠心病心肌缺血血瘀证小型猪血清 NMR 代谢组学的研究分析[J]. 化学研究简报, 2011, 39(8): 1274-1278. Wang Yong, Li Zhongfeng, Chen Jianxin, et al. Study of mini-pigs serum of coronary heart disease (chronic myocardial ischemia) with syndrome of blood stasis based on nuclear magnetic resonance metabolomics[J]. Chinese Journal of Analytical Chemistry, 2011, 39(8): 1274-1278.
[8] Bonuccelli G, Tsirigos A, Whitaker-Menezes D, et al. Ketones and lactate "fuel" tumor growth and metastasis:Evidence that epithelial cancer cells use oxidative mitochondrial metabolism[J]. Cell Cycle, 2010, 9(17): 3506-3514.
[9] Lim H Y, Ho Q, Low J, et al. Respiratory competent mitochondria in human ovarian and peritoneal cancer[J]. Mitochondrion, 2011, 11(3): 437-443.
[10] Pavlides S, Whitaker-Menezes D, Castello-Cros R, et al. The reverse Warburg effect: Aerobic glycolysis in cancerassociated fibroblasts and the tumor stroma[J]. Cell Cycle, 2009, 8(23): 3984-4001.
[11] 孙敏, 王海洋, 徐瑾. 基于代谢组学的胃癌组织和血浆的糖代谢研究[J]. 胃肠病学, 2011, 16(10): 610-612. Sun Min, Wang Haiyang, Xu Jin, et al. Study on saccharometabolism of gastric cancer tissues and plasma based upon metabolomics[J]. Chinese Journal of Gastroenterology, 2011, 16(10): 610-612.
[12] 杜芳, 王立东, 齐义军, 等. 利用肿瘤相关抗原微阵列检测食管和贲门癌及癌前病变患者血清中多个自身抗体的变化特征[J]. 中华肿瘤防治杂志, 2006, 13(17): 1289-1292. Du Fang, Wang Lidong, Qi Yijun, et al. Detection of multiple serum autoantibody in patients with esophageal and gastric cardia precancerous and cancerous lesion using tumor-associated antigens min-i array[J]. Chinese Journal of Cancer Prevention and Treatment, 2006, 13 (17): 1289-1292.
[13] 刘保池, 王立东, 杜芳, 等. 肿瘤相关抗原与自身抗体检测对食管癌早期发现和高危人群预警的意义[J]. 中华肿瘤防治杂志, 2007, 14 (9): 645-649. Liu Baochi, Wang Lidong, Du Fang, et al. Clinical significance of tumor-associated antigens and autoantibody measurement in high-risk subject screening and early detection of esophageal cancer[J]. Chinese Journal of Cancer Prevention and Treatment, 2007, 14(9): 645-649.
[14] 陈辑. 前列腺癌组织胆碱摄取值与其肿瘤生物学行为相关性的初步研究[D]. 济南: 山东大学, 2012. Chen Ji. Study on the relationship between choline uptake of prostate cancer and its biological behavior[D]. Jinan: Shandong University, 2012.
[15] 常林凤, 卢光明, 朱虹, 等. 11C-胆碱与18氟去氧葡萄糖PET/CT显像在肺癌诊断中的对比研究[J]. 医学研究生学报, 2008, 21(8): 27-28. Chang Linfeng, Lu Guangming, Zhu Hong, et al. Comparison of 11Ccholine and 18FDG PET/CT imaging in the diagnosis of pulmonary carc inoma[J]. Journal of Medical Postgraduates, 2008, 21(8): 27-28.
[16] 李昕. 11C-胆碱PET/CT显像在前列腺癌及肺癌中的应用价值[D]. 济南: 山东大学, 2010. Li Xin. The value of 11C-choline PET/CT imagingon prostate cancer and lung caner[D]. Jinan: Shandong University, 2010.
[17] 魏源. 补充肌酸对运动能力和健康的影响[J]. 沈阳体育学院学报, 2005, 24(2): 11-14. Wei Yuan. Influences of supplying creatine on sports ability and health[J]. Journal of Shenyang Physical Education Institute, 2005, 24(2): 11-14.
[18] Meyer R A, Sweeney H L, Kushmerick M J. A simple analysis of the phosphocreatine[J]. American Journal of Physiology, 1984, 246(5Pt1): C365-C377.
[19] 郭瑞霞, 乔玉环. 磷脂酰肌醇3激酶/蛋白激酶B信号传导与妇科恶性肿瘤关系研究[J]. 中华妇产科杂志, 2005, 40(5): 358-360. Guo Ruixia, Qiao Yuhuan. Study the relationship between phosphatidylinositol 3 kinase/Protein kinase B signal pathway and gynecologic malignant tumor[J]. Chinese Journal of Obstetrics and Gynecology, 2005, 40(5): 358-360.
[20] 徐卫芳, 哈木拉提·吾甫尔, 李凤森. 基于代谢组学分析COPD肾虚痰瘀证与异常黑胆质证共性特征[J]. 科技导报, 2013, 31(23): 5-11. Xu Weifang, Upur Halmurat, Li Fengsen. Metabonomics common characteristics of COPD about traditional Chinese medicine kidney depletion-phlegm-stasis syndrome and uygur medicine abnormal savda syndrome[J]. Science & Technology Review, 2013, 31(23): 5-11.
[21] 巴吐尔·买买提明, 哈木拉提·吾甫尔, 豪富华, 等. 异常黑胆质型与非异常黑胆质型肿瘤患者血浆代谢组学差异分析[J]. 科技导报, 2009, 27(20): 39-43. Mamtimin Batur, Upur Halmurat, Hao Fuhua, et al. Metabonomic differences of plasma between abnormal savda and unsavda syndrome neoplasm patients[J]. Science & Technology Review, 2009, 27(20): 39-43.
