This paper studies the protective effect of pine pollen on renal damage in a mouse model of diabetic nephropathy and its mechanism. Fifty adult male C57BL/6J mice were used, and the mouse model of diabetic nephropathy was induced by streptozotocin (STZ). The mice were randomly divided into the control group (n=10) and the STZ model group (n=40). The latter received intraperitoneal injection of a single high dose of 150 mg body weight of STZ dissolved in citrate buffer to induce diabetic nephropathy. The control group were injected with sodium citrate buffer alone. Glucose concentrations in the tail vein blood of the mice were measured 1 week after STZ administration. Only STZ-injected mice with fasting blood glucose concentrations >11.1 mmol/L were included in the diabetic groups. After that, blood glucose and urine protein were detected in 24 hours regularly, and mice with continuing albuminuria were regarded as those with diabetic nephropathy, which totaled 28. The 28 mice were randomly divided into the STZ model group (n=14) and the pine pollen group (n=14). Mice in the pine pollen group were given pine pollen (750 mg) daily. Meanwhile, enema was performed using equal volume of normal saline in the control and STZ model groups. 7 weeks later, plasma glucose concentration, 24 h urinary protein excretion, urine creatinine, serum creatinine and plasma urea nitrogen were detected. The renal tissue was collected to assay the renal weight/body weight ratio and relative level of MDA. Masson staining and Silver staining were performed for histological analyses. The expression of p-p38 and p-ERK in renal cortical tissues was examined using Western Blot. Compared with that in the STZ model group, pine pollen apparently ameliorated renal functional lesion, reduced the renal weight/ body weight ratio and extracellular matrix accumulation. Pine pollen can also reduce the level of MDA and inhibit the activation of p38 and ERK in diabetic kidney. The results showed that pine pollen may reduce oxidative damage of the kidney and extracellular matrix accumulation by inhibiting the phosphorylation level of p38 and ERK in renal cortical tissues.
LI Nan
,
FENG Zhenzhong
,
ZHAO Yan
,
GU Congyou
,
ZHU Bo
,
OU Yurong
. Protective Effect of Pine Pollen on Mice Renal Damage in a Model of Diabetic Nephropathy[J]. Science & Technology Review, 2014
, 32(4-5)
: 95
-99
.
DOI: 10.3981/j.issn.1000-7857.2014.h1.016
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