探讨维药异常黑胆质成熟剂（ASM）抗肿瘤作用及其对肿瘤细胞迁移的影响。体外培养人肝癌Bel-7402细胞株、人乳腺癌BCAP细胞株、人宫颈癌Hela细胞株、人胃癌BGC-823细胞株，采用四甲基偶氮唑蓝（tetrzolium-based colorimetric assay，MTT）法检测不同浓度ASM（1、2.5、5、10、15、20、50mg/mL）对人肝癌Bel-7402细胞株、人乳腺癌BCAP细胞株、人宫颈癌Hela细胞株、人胃癌BGC-823细胞株4种肿瘤细胞增殖的影响；选择人肝癌Bel-7402细胞株为研究对象，采用细胞划痕运动实验和扫描电镜技术，观察不同浓度ASM对肝癌细胞迁移的影响。MTT检测结果显示，ASM对人肝癌Bel-7402细胞株、人乳腺癌BCAP细胞株、人宫颈癌Hela细胞株、人胃癌BGC-823细胞株生长均有一定抑制作用，且在15~20mg/mL作用下最为明显，对不同肿瘤细胞株抑制作用不一；体外细胞划痕运动实验和扫描电镜结果显示，ASM对人肝癌Bel-7402细胞运动有一定抑制作用。由此推论，ASM对肿瘤细胞增殖和肿瘤细胞迁移有一定抑制作用。
The possible mechanism of Uighur medicine Abnormal Savda Munzip (ASM) on the anti-tumor effects and its influence on tumor cell migration are explored. MTT was applied to determine the cell proliferation of human hepatoma (Bel-7402), human breast carcinoma (BCAP), human cervical cancer (Hela), and human gastric cancer proliferation (BGC-823) after treated with different concentration of ASM (1, 2.5, 5, 10, 20, 50mg/mL) for 48h; Human hepatoma cell line (Bel-7402) was selected for the cell motility study, the cell migration influence on human hepatoma (Bel-7402) were assessed by the wound-healing assay and the Scanning Electron Microscopy (SEM) technique after treated with ASM (5, 10, 20, 50mg/mL) for 48h. ASM produced the cytotoxinic effect on these four type of tumor cells in vitro and all type cells showed the obviously vitro inhibition after treated with ASM (15, 20mg/mL), there were difference inhibited effect among the four type of tumor cells, the results of wound-healing assay with SEM show that the moving ability of Bel-7402 treated with ASM has decreased. It is deduced that ASM might inhibit the ability of proliferation and moving of tumor cell.
