专题:2023年科技热点回眸

2023年小麦新基因挖掘和遗传改良新技术研究回眸

  • 叶兴国 ,
  • 林志珊 ,
  • 王轲 ,
  • 唐华丽 ,
  • 韩志阳
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  • 中国农业科学院作物科学研究所, 北京 100081
叶兴国,研究员,研究方向为小麦生物技术育种,电子信箱:yexingguo@caas.cn

收稿日期: 2023-12-18

  修回日期: 2023-12-28

  网络出版日期: 2024-04-09

基金资助

国家自然科学基金项目(32272180)

Annual progress on new gene characterization and novel technology development for wheat genetic improvement in 2023

  • YE Xingguo ,
  • LIN Zhishan ,
  • WANG Ke ,
  • TANG Huali ,
  • HAN Zhiyang
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  • Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2023-12-18

  Revised date: 2023-12-28

  Online published: 2024-04-09

摘要

2023年小麦生物育种相关研究取得了重要进展:挖掘了调控小麦高产、抗病、抗旱、耐低温等性状相关的新基因,创制了具有高产、抗病、优质等性状的新种质,构建了大批量EMS突变体库,完善了利用引导编辑系统编辑小麦内源基因和诱导系诱导小麦单倍体的技术体系,丰富了小麦基因组测序数据的覆盖度和多态性。今后需要注重这些新基因、新种质、新技术和新测序数据在小麦育种中的应用,充分发挥它们的作用。尤其是尽可能将多个优良基因或性状进行聚合,培育兼具高产、多抗、优质等多个优良性状的小麦品种。同时,加强小麦抗蚜虫、耐高温和高光效等性状的研究,努力解决小麦生产中的实际问题。

本文引用格式

叶兴国 , 林志珊 , 王轲 , 唐华丽 , 韩志阳 . 2023年小麦新基因挖掘和遗传改良新技术研究回眸[J]. 科技导报, 2024 , 42(1) : 174 -187 . DOI: 10.3981/j.issn.1000-7857.2024.01.011

Abstract

In the past year, dramatic progress on wheat genetic improvement was achieved, including the exploring of new genes related to high yield, disease resistance, and drought and low temperature tolerance, the development of new germplasms with ideal disease resistance and promising yield and good quality potential, the creating of EMS-induced mutants in a large scale,the improvement of prime editing technology used in wheat and haploid induction system by wheat edited mutant, and the enrichment of wheat genome sequencing data on coverage and polymorphism. In future studies, these genes, materials,technologies, and genome sequencing information should be better used in wheat breeding for fully playing their roles.Particularly, multiple available genes or traits can be pyramided by crossing or advanced techniques to develop ideal wheat varieties which have high yield, multiple resistances, and satisfying quality. Additionally, more researches on aphid resistance,high temperature tolerance, and high photosynthetic efficiency are necessary in this crop for reducing yield loss in wheat production.

参考文献

[1] Li H, Hua L, Zhao S, et al. Cloning of the broad-spectrum wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides[J]. Nature Communications, 2023,14(1):6072.
[2] Wang Y, Abrouk M, Gourdoupis S, et al. An unusual tandem kinase fusion protein confers leaf rust resistance in wheat[J]. Nature Genetics, 2023, 55(6):914-920.
[3] Ni F, Zheng Y, Liu X, et al. Sequencing trait-associated mutations to clone wheat rust-resistance gene YrNAM[J].Nature Communications, 2023, 14(1):4353.
[4] Yu G, Matny O, Gourdoupis S, et al. The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase[J]. Nature Genetics, 2023, 55(6):921-926.
[5] Zhang J, Nirmala J, Chen S, et al. Single amino acid change alters specificity of the multi-allelic wheat stem rust resistance locus Sr9[J]. Nature Communications,2023, 14(1):7354.
[6] Han G, Liu H, Zhu S, et al. Two functional CC-NBSLRR proteins from rye chromosome 6RS confer differential age-related powdery mildew resistance to wheat[J/OL]. Plant Biotechnology Journal, 2023.[2023-12-18].https://doi.org/10.1111/pbi.14165.
[7] Li Y, Wei Z Z, Sela H, et al. Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69[J/OL].Plant Communications, 2023.[2023-12-18]. https://doi.org/10.1016/j.xplc.2023.100646.
[8] Manser B, Zbinden H, Herren G, et al. Wheat zinc finger protein TaZF interacts with both the powdery mildew AvrPm2 protein and the corresponding wheat Pm2a immune receptor[J/OL]. Plant Communications, 2023.[2023-12-18]. https://doi.org/10.1016/j.xplc.2023.100769.
[9] Mishina K, Suzuki T, Oono Y, et al. Wheat Ym2 originated from Aegilops sharonensis and confers resistance to soil-borne Wheat yellow mosaic virus infection to the roots[J]. Proceedings of the National Academy of Sciences, 2023, 120(11):e2214968120.
[10] Liu P, Shi C, Liu S, et al. A papain-like cysteine protease-released small signal peptide confers wheat resistance to wheat yellow mosaic virus[J]. Nature Communications, 2023, 14(1):7773.
[11] Wang L, Zhang K, Wang Z, et al. Appropriate reduction of importin-α gene expression enhances yellow dwarf disease resistance in common wheat[J/OL]. Plant Biotechnology Journal, 2023.[2023-12-18]. https://doi.org/10.1111/pbi.14204.
[12] Song L, Liu J, Cao B, et al. Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat[J]. Nature, 2023, 617(7959):118-124.
[13] Zhang J, Zhang Z, Zhang R, et al. Type I MADS-box transcription factor TaMADS-GS regulates grain size by stabilizing cytokinin signalling during endosperm cellularization in wheat[J]. Plant Biotechnology Journal,2023, 13(10):1076.
[14] Li X, Cao B, Du D, et al. TaACTIN7-D regulates plant height and grain shape in bread wheat[J]. Journal of Genetics and Genomics, 2023, 50(11):895-908.
[15] Dong C, Zhang L, Zhang Q, et al. Tiller number1 encodes an ankyrin repeat protein that controls tillering in bread wheat[J]. Nature Communications, 2023, 14(1):836.
[16] Kong X, Wang F, Wang Z, et al. Grain yield improvement by genome editing of TaARF12 that decoupled peduncle and rachis development trajectories via differential regulation of gibberellin signalling in wheat[J]. Plant Biotechnology Journal, 2023, 21(10):1990-2001.
[17] Xie Z, Zhang L, Zhang Q, et al. A Glu209Lys substitution in DRG1/TaACT7, which disturbs F-actin organization, reduces plant height and grain length in bread wheat[J]. New Phytologist, 2023, 240(5):1913-1929.
[18] Liu H, Si X, Wang Z, et al. TaTPP-7A positively feedback regulates grain filling and wheat grain yield through T6P-SnRK1 signalling pathway and sugar-ABA interaction[J]. Plant Biotechnology Journal, 2023, 21(6):1159-1175.
[19] Yan Q, Lu Y, Pang Y, et al. TaCRTISO dosage modulates plant height and spike number per plant in wheat[J/OL]. Plant Physiology, 2023.[2023-12-18]. https://doi.org/10.1073/pnas.2214968120.
[20] Tai L, Wu J, Jing Y, et al. A genome-wide association study uncovers that TaPI4K-2A regulates pre-harvest sprouting in wheat[J/OL]. Plant Communications, 2023.[2023-12-18]. https://doi.org/10.1016/j.xplc.2023.1007-39.
[21] Liu X, Bie X M, Lin X, et al. Uncovering the transcriptional regulatory network involved in boosting wheat regeneration and transformation[J]. Nature Plants, 2023, 9(6):908-925.
[22] Yu Y, Yu H, Peng J, et al. Enhancing wheat regeneration and genetic transformation through overexpression of TaLAX1[J/OL]. Plant Communications, 2023.[2023-12-18]. https://doi.org/10.1016/j.xplc.2023.100738.
[23] Zhang R, Zhang S, Li J, et al. CRISPR/Cas9-targeted mutagenesis of TaDCL4, TaDCL5 and TaRDR6 induces male sterility in common wheat[J]. Plant Biotechnology Journal, 2023, 21(4):839-853.
[24] Xu L, Tang Y, Yang Y, et al. Microspore expressed SCULP1 is required for p-coumaroylation of sporopollenin, exine integrity, and pollen development in wheat[J].New Phytologist, 2023, 239(1):102-115.
[25] Zhang L, Zhang N, Wang S, et al. A TaSnRK1α Modulates TaPAP6L-Mediated wheat cold tolerance through regulating endogenous jasmonic acid[J]. Advanced Science, 2023, 10(31):2303478.
[26] Tian G, Wang S, Wu J, et al. Allelic variation of TaWD40-4B.1 contributes to drought tolerance by modulating catalase activity in wheat[J]. Nature Communications, 2023, 14(1):1200.
[27] Wang J, Li C, Li L, et al. DIW1 encoding a clade I PP2C phosphatase negatively regulates drought tolerance by dephosphorylating TaSnRK1.1 in wheat[J]. Journal of Integrative Plant Biology, 2023, 65(8):1918-1936.
[28] Ma J, Geng Y, Liu H, et al. TaTIP41 and TaTAP46 positively regulate drought tolerance in wheat by inhibiting PP2A activity[J]. Journal of Integrative Plant Biology,2023, 65(9):2056-2070.
[29] Wang Z, Zhang Y, Kang Z, et al. Improvement of wheat drought tolerance through editing of TaATX4 by CRISPR/Cas9[J]. Journal of Genetics and Genomics, 2023, 50(11):913-916.
[30] Li S M, Zhang Y F, Liu Y L, et al. The E3 ligase TaGW2 mediates transcription factor TaARR12 degradation to promote drought resistance in wheat[J/OL]. Plant Cell, 2023.[2023-12-18]. https://doi.org/10.1093/plcell/koad307.
[31] Guo X, Shi Q, Wang M, et al. Functional analysis of the glutathione S-transferases from Thinopyrum and its derivatives on wheat Fusarium head blight resistance[J].Plant Biotechnology Journal, 2023, 21(6):1091-1093.
[32] Xu S S, Lyu Z F, Zhang N, et al. Genetic mapping of the wheat leaf rust resistance gene Lr19 and development of translocation lines to break its linkage with yellow pigment[J]. Theoretical and Applied Genetics, 2023,136(9):200.
[33] Qiu Y L, Han Z Y, Liu N T, et al. Effects of Aegilops longissima chromosome 1Slon wheat bread-making quality in two types of translocation lines[J]. Theoretical and Applied Genetics, 2023, 137(1):2.
[34] Huang J, Lin Q, Fei H, et al. Discovery of deaminase functions by structure-based protein clustering[J]. Cell,2023, 186(15):3182-3195.
[35] Hu J, Sun Y, Li B, et al. Strand-preferred base editing of organellar and nuclear genomes using CyDENT[J/OL].Nature Biotechnology, 2023.[2023-12-18]. https://doi.org/10.1038/s41587-023-01910-9.
[36] Xiong X, Liu K, Li Z, et al. Split complementation of base editors to minimize off-target edits[J]. Nature Plants, 2023, 9(11):1832-1847.
[37] Zhong Z, Liu G, Tang Z, et al. Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system[J]. Nature Communications, 2023, 14(1):6102.
[38] Ni P, Zhao Y, Zhou X, et al. Efficient and versatile multiplex prime editing in hexaploid wheat[J]. Genome Biology, 2023, 24(1):156.
[39] Tang H, Wang K, Zhang S, et al. A fast technique for visual screening of wheat haploids generated from TaMTLedited mutants carrying anthocyanin markers[J]. Plant Communications, 2023, 4(3):100569.
[40] Qi X, Guo S, Zhong Y, et al. Establishment of an efficient haploid identification system by engineering anthocyanin accumulation in the wheat embryo[J]. Plant Communications, 2023, 4(3):100568.
[41] Jia J, Zhao G, Li D, et al. Genome resources for the elite bread wheat cultivar Aikang 58 and mining of elite homeologous haplotypes for accelerating wheat improvement[J]. Molecular Plant, 2023, 16(12):1893-1910.
[42] Niu J, Ma S, Zheng S, et al. Whole-genome sequencing of diverse wheat accessions uncovers genetic changes during modern breeding in China and the United States[J]. The Plant Cell, 2023, 35(12):4199-4216.
[43] Ahmed H I, Heuberger M, Schoen A, et al. Einkorn genomics sheds light on history of the oldest domesticated wheat[J]. Nature, 2023, 620(7975):830-838.
[44] Wang X F, Li H, Shen T, et al. A near-complete genome sequence of einkorn wheat provides insight into the evolution of wheat a subgenomes[J]. Plant Communications, 2023, 16:100768.
[45] Li H, Zhu L, Fan R, et al. A platform for whole-genome speed introgression from Aegilops tauschii to wheat for breeding future crops[J/OL]. Nature Protocols, 2023.[2023-12-18]. https://doi.org/10.1038/s41596-023-009-22-8.
[46] Xiong H, Guo H, Fu M, et al. A large-scale wholeexome sequencing mutant resource for functional genomics in wheat[J]. Plant Biotechnology Journal, 2023, 21(10):2047-2056.
[47] Wang D, Li Y, Wang H, et al. Boosting wheat functional genomics via an indexed EMS mutant library of KN9204[J]. Plant Communications, 2023, 4(4):100593.
[48] Wang W Q, Guan X Z, Gan Y, et al. Creating large EMS populations for functional genomics and breeding in wheat[J/OL]. Journal of Integrative Agriculture, 2023.[2023-12-18]. https://doi.org/10.1016/j.jia.2023.05.039.
[49] Wen J, Qin Z, Sun L, et al. Alternative splicing of TaHSFA6e modulates heat shock protein-mediated translational regulation in response to heat stress in wheat[J]. New Phytologist, 2023, 239(6):2235-2247.
[50] Wang H, Feng M, Jiang Y, et al. Thermosensitive SUMOylation of TaHsfA1 defines a dynamic ON/OFF molecular switch for the heat stress response in wheat[J].The Plant Cell, 2023, 35(10):3889-3910.
[51] Niu K X, Chang C Y, Zhang M Q, et al. Suppressing ASPARTIC PROTEASE1 prolongs photosynthesis and increases wheat grain weight[J]. Nature Plants, 2023, 9(6):965-977.
[52] Dong W, Chang T, Dai H, et al. Creating a C4-like vein pattern in rice by manipulating short root and auxin levels[J/OL]. Science Bulletin, 2023.[2023-12-18]. https://doi.org/10.1016/j.scib.2023.10.005.
[53] Meers C, Le H C, Pesari S R, et al. Transposon-encoded nucleases use guide RNAs to promote their selfish spread[J]. Nature, 2023, 622(7984):863-871.
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