专题论文

小麦抗穗发芽分子标记开发及育种应用

  • 张海萍 ,
  • 常成 ,
  • 司红起 ,
  • 卢杰 ,
  • 马传喜
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  • 安徽农业大学农学院, 农业部黄淮南部小麦生物学与遗传育种重点实验室, 合肥 230036
张海萍,副教授,研究方向为小麦抗逆分子育种,电子信箱:zhhp20@163.com

收稿日期: 2016-08-29

  修回日期: 2016-11-05

  网络出版日期: 2016-12-13

基金资助

国家现代农业产业技术体系建设专项(CARS-3-1);农业部公益性行业科技专项(201203033-04);国家自然科学基金项目(31401372);安徽省自然科学基金项目(1408085MC60)

Developing of molecular marker for pre-harvest sprouting resistance and its application in wheat MAS breeding

  • ZHANG Haiping ,
  • CHANG Cheng ,
  • SI Hongqi ,
  • LU Jie ,
  • MA Chuanxi
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  • College of Agronomy, Anhui Agricultural University; Key Laboratory of Wheat Biology and Genetic Improvement in South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036, China

Received date: 2016-08-29

  Revised date: 2016-11-05

  Online published: 2016-12-13

摘要

综述了小麦收获前穗发芽的影响因素和应对策略、穗发芽抗性的QTL/基因研究进展、开发的分子标记及其育种应用,总结了利用分子标记开展抗穗发芽分子标记辅助育种取得的成效。结果表明,温度和降雨是影响小麦穗发芽发生的主要环境因素,培育抗穗发芽品种是解决该问题的有效途径。

本文引用格式

张海萍 , 常成 , 司红起 , 卢杰 , 马传喜 . 小麦抗穗发芽分子标记开发及育种应用[J]. 科技导报, 2016 , 34(22) : 81 -86 . DOI: 10.3981/j.issn.1000-7857.2016.22.011

Abstract

This review focuses on the major factors affecting wheat pre-harvest sprouting (PHS), the measures to solve the wheat PHS problems, the recent advances in QTL/gene for wheat PHS resistance, and the identification of the molecular marker for the PHS tolerance and its application in the wheat MAS breeding made by our research group in recent years. The results reveal that the temperature and the rainfalls are the major factors affecting the wheat PHS in the field, and the development of wheat varieties with the PHS resistance can effectively solve this problem. Using different mapping populations, several major QTLs and molecular markers for the wheat PHS resistance are identified.

参考文献

[1] Xiao S H, Zhang X Y, Yan C S, et a1. Germplasm improvement for pre-harvest sprouting resistance in Chinese white ograined wheat:An over-view of the current strategy[J]. Euphytica, 2002, 126:35-38.
[2] Groos C, Gay G, Perretant R M, et a1. Study of the relationship be-tween pre-harvest sprouting and grain color by quantitative trait loci analysis in a white red grain bread-wheat cross[J]. Theoretical and Ap-plied Genetics, 2002, 104:39-47.
[3] 肖世和, 闫长生, 张海萍, 等. 小麦穗发芽研究[M]. 北京:中国农业科学技术出版社, 2004:1-17. Xiao Shihe, Yan Changsheng, Zhang Haiping, et al. Study on pre-har-vest sprouting in wheat[M]. Beijing:China Agricultural Science and Technology Publishing House, 2004:1-17.
[4] 高剑波, 潘建梅, 陆钦武, 等. 小麦田间穗发芽的影响因素及防止对策[J]. 种子科技, 2006(3):39-41. Gao Jianbo, Pan Jianmei, Lu Qinwu, et al. Influence factors of wheat sprout in field and its prevention measures[J]. Seed Technology, 2006(3):39-41.
[5] 朱美荣, 张如标, 王蓓蓓, 等. 小麦穗发芽生理及调控途径研究进展[J]. 金陵科技学院学报, 2010, 26(2):50-54. Zhu Meirong, Zhang Rubiao, Wang Beibei, et al. Wheat pre-harvest sprouting physiology and control approaches[J]. Journal of Jinling Insti-tute of Technology, 2010, 26(2):50-54.
[6] Ueno K. Effects of desiccation and a change in temperature on germina-tion of immature grains of wheat[J]. Euphytica, 2002, 126:107-113.
[7] 姚金保, 钱存鸣, 周朝飞, 等. 白皮小麦抗穗发芽性的鉴定及抗性机理的探讨[J]. 江苏农业科学, 1996(4):17-19. Yao Jinbao, Qian Cunming, Zhou Chaofei, et al. Discussion on pre-har-vest sprouting resistance mechanism in white-grained wheat variety[J]. Jiangsu Agricultural Sciences, 1996(4):17-19.
[8] Gao X, Hu C H, Li H Z, et al. Factors affecting pre-harvest sprouting resistance in wheat (Triticum aestivum L.):A review[J]. The Journal of Animal & Plant Sciences, 2013, 23(2):556-565.
[9] Rehman Arif M A, Neumann K, Nagel M, et al. An association map-ping analysis of dormancy and pre-harvest sprouting in wheat[J]. Eu-phytica, 2012, 188:409-417.
[10] Kulwal P. Ishikawa G, Benscher D. et al. Association mapping for preharvest sprouting resistance in white winter wheat[J]. Theoretical and Applied Genetics, 2012, 125:793-805.
[11] Kulwal P L, Kumar N, Gaur A, et al. Mapping of a major QTL for pre-harvest sprouting tolerance on chromosome 3A in bread wheat[J]. Theo-retical and Applied Genetics, 2005, 111:1052-1059.
[12] Mohan A, Kulwal P, Singh R, et al. Genome-wide QTL analysis for pre-harvest sprouting tolerance in bread wheat[J]. Euphytica, 2009, 168:319-329.
[13] Osa M, Kato K, Mori M, et al. Mapping QTLs for seed dormancy and the Vp1 homologue on chromosome 3A in wheat[J]. Theoretical and Applied Genetics, 2003, 106:1491-1496.
[14] Mori M, Uchino N, Chono M, et al. Mapping QTLs for grain dormancy on wheat chromosome 3A and group 4 chromosomes, and their com-bined effect[J]. Theoretical and Applied Genetics, 2005, 110:1315-1323.
[15] Liu S B, Cai S B, Robert G, et al. Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco[J]. Theoretical and Applied Genetics, 2008, 117:691-699.
[16] Liu S B, Bai G H. Dissection and fine mapping of a major QTL for preharvest sprouting resistance in white wheat Rio Blanco[J]. Theoreti-cal and Applied Genetics, 2010, 121:1395-1404.
[17] Nakamura S, Abe F, Kawahigashi H, et al. A wheat homolog of moth-er of FT and TFL1 acts in the regulation of germination[J]. Plant Cell, 2011, 23:3215-3229.
[18] Liu S B, Sehgal S K, Li J R, et al. Cloning and characterization of a critical regulator for pre-harvest sprouting in wheat[J]. Genetics, 2013, 195:263-273.
[19] Sears E R. Cytogenetic studies with polyploidy species of wheat. II. Additional chromosome aberrations in Triticum vulgare[J]. Genetics, 1944, 29:232-246.
[20] Himi E, Maekawa M, Miura H, et al. Development of PCR markers for Tamyb10 related to R-1, red grain color gene in wheat[J]. Theoretical and Applied Genetics, 2011, 122:1561-1576.
[21] Himi E, Maekawa M, Matsuura T, et al. Real-time PCR-mediated di-agnosis of hemizygosity at the Tamyb10-D1 locus controlling grain col-or in wheat[J]. Mol Breeding, 2015, 35:90-99.
[22] Bi H H, Sun Y W, Xiao Y G, et al. Characterization of DFR allelic variations and their associations with pre-harvest sprouting resistance in a set of red-grained Chinese wheat germplasm[J]. Euphytica, 2014, 195:197-207.
[23] Bailey P C, McKibbin R S, Lenton J R, et al. Genetic map locations for orthologous Vp1 genes in wheat and rice[J]. Theoretical and Ap-plied Genetics, 1999, 98:281-284.
[24] Yang Y, Zhao X L, Xia L Q, et al. Development and validation of a Viviparous-1 STS marker for preharvest sprouting tolerance in Chi-nese wheats[J]. Theoretical and Applied Genetics, 2007, 115:971-980.
[25] Chang C, Zhang H P, Feng J M, et al. Identifying alleles of Vivipa-rous-1B associated with pre-harvest sprouting in micro-core collec-tions of Chinese wheat germplasm[J]. Molecular Breeding, 2010, 25:481-490.
[26] Chang C, Zhang H P, Zhao Q X, et al. Rich allelic variations of Vivip-arous-1A and their associations with seed dormancy/pre-harvest sprouting of common wheat[J]. Euphytica, 2011, 179:343-353.
[27] Kato K, Nakamura W, Tabiki T, et al. Detection of loci controlling seed dormancy on group 4 chromosomes of wheat and comparative mapping with rice and barley genomes[J]. Theoretical and Applied Ge-netics, 2001, 102:980-985.
[28] Torada A, Ikeguchi S, Koike M. Mapping and validation of PCRbased markers associated with a major QTL for seed dormancy in wheat[J]. Euphytica, 2005, 143:251-255.
[29] Mares D J, Rathjen J, Mrva K, et al. Genetic and environmental con-trol of dormancy in white-grained wheat (Triticum aestivum L.)[J]. Eu-phytica, 2009, 168:311-318.
[30] Chen C X, Cai S B, Bai G H, et al. A major QTL controlling seed dor-mancy and pre-harvest sprouting resistance on chromosome 4A in a Chinese wheat landrace[J]. Molecular Breeding, 2008, 21:351-358.
[31] Liu S B, Bai G H, Cai S B, et al. Dissection of genetic components of preharvest sprouting resistance in white wheat[J]. Molecular Breeding, 2011, 27:511-523.
[32] Barrero J M, Cavanagh C, Verbyla K L, et al. Transcriptomic analysis of wheat near-isogenic lines identifies PM19-A1 and A2 as candi-dates for a major dormancy QTL[J]. Genome Biology, 2015, 16:93-111.
[33] Munkvold J D, Tanaka J, Benscher D, et al. Mapping quantitative trait loci for preharvest sprouting resistance in white wheat[J]. Theoretical and Applied Genetics, 2009, 119:1223-1235.
[34] Somyong S, Ishikawa G, Munkvold J D, et al. Fine mapping of a pre-harvest sprouting QTL interval on chromosome 2B in white wheat[J]. Theoretical and Applied Genetics, 2014, 127:1843-1855.
[35] Zhang Y J, Miao X L, Xia X C, et al. Cloning of seed dormancy genes (TaSdr) associated with tolerance to pre-harvest sprouting in common wheat and development of a functional marker[J]. Theoretical and Ap-plied Genetics, 2014, 127:855-866.
[36] 张海萍, 冯继明, 常成, 等. 中国小麦地方品种籽粒强休眠特性的主效基因鉴定[J]. 农业生物技术学报, 2011, 19(2):270-277. Zhang Haiping, Feng Jiming, Chang Cheng, et al. Investigation of ma-jor loci contributing to strong seed dormancy of Chinese wheat landra-ce[J]. Journal of Agricultural Biotechnology, 2011, 19(2):270-277.
[37] 黎裕, 王健康, 邱丽娟, 等. 中国作物分子育种现状与发展前景[J]. 作物学报, 2010, 36(9):1425-1430. Li Yu, Wang Jiankang, Qiu Lijuan, et al. Crop molecular breeding in China:Current status and perspectives[J]. Acta Agronomica Sinica, 2010, 36(9):1425-1430.
[38] Bernardo R. Molecular marker and selection for complex traits in plants:Learning from the last 20 years[J]. Crop Science, 2008, 48:1649-1664.
[39] Kottearachchi N S, Uchino N, Kato K, et al. Increased grain dormancy in white-grained wheat by introgression of preharvest sprouting toler-ance QTLs[J]. Euphytica, 2006, 152:421-428.
[40] Hickey L T, Dieters M J, DeLacy I H, et al. Screening for grain dor-mancy in segregating generations of dormant 3 non-dormant crosses in white-grained wheat (Triticum aestivum L.)[J]. Euphytica, 2010, 172:183-195.
[41] Singh R, Hucl P, Baga M, et al. Validation of molecular markers for pre-harvest sprouting resistance in bread wheat[J]. Cereal Research Communications, 2012, 40(2):194-203.
[42] Graybosch R A, Amand P S, Bai G H. Evaluation of genetic markers for prediction of preharvest sprouting tolerance in hard white winter wheat[J]. Plant Breeding, 2013, 132:359-366.
[43] Xiao S H, Zhang H P, You G X, et al. Integration of marker-assisted selection for resistance to pre-harvest sprouting with selection for grain-filling rate in breeding of white-kernelled wheat for the Chinese environment[J]. Euphytica, 2012, 188:85-88.
[44] 张海萍, 常成, 游光霞, 等. 中国小麦微核心种质及地方品种籽粒休眠特性的分子标记鉴定[J]. 作物学报, 2010, 36(10):1649-1656. Zhang Haiping, Chang Cheng, You Guangxia, et al. Identification of molecular markers associated with seed dormancy in mini core collec-tions of Chinese wheat and landraces[J]. Acta Agronomica Sinica, 2010, 36(10):1649-1656.
[45] Lu J, Chang C, Zhang H P, et al. Identification of a novel allele of TaCKX6a02 associated with grain size, filling rate and weight of com-mon wheat[J]. PLoS One, 2015. doi:10.1371/journal.pone.0144765.
[46] Chang C, Lu J, Zhang H P, et al. Copy number variation of cytokinin oxidase gene Tackx4 associated with grainweight and chlorophyll con-tent of flag leaf in common wheat[J]. PLoS One, 2015. doi:10.1371/journal.pone.0145970.
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