为探讨蜂胶黄酮(Pinobanksin-3-acetate, PB3A)对结肠癌HCT-116 细胞增殖及细胞凋亡的影响, 采用四甲基偶氮唑盐(MTT)比色法, 检测不同浓度、不同时间PB3A、5-氟尿嘧啶(5-FU)及两种药物联合作用对HCT-116 细胞生长所产生的影响, 倒置显微镜观察细胞的形态学变化特点, Annexin V-FITC/PI 双染色、流式细胞仪检测药物作用24 h 后的细胞凋亡率。结果显示, PB3A 对HCT-116 细胞增殖有明显的抑制作用, 并呈浓度和时间依赖性。其抑制活性与5-FU 几乎没有显著性差异, 联合用药具有协同效应; 在一定剂量范围内, 可见凋亡细胞明显增多。流式细胞仪分析结果表明, 细胞凋亡率明显上升, 呈剂量依赖性。PB3A 对HCT-116 细胞具有明显的增殖抑制和诱导细胞凋亡作用。
The Pinobanksin-3-acetate (PB3A) is a natural flavonoid commonly present in propolis, honey, and many plants. The purpose of this study is to evaluate the anticancer activity of the PB3A on the human colon cancer in vitro. The effect of different concentrations of PB3A, 5-fluorouracil (5-FU) and a combination of PB3A and 5-FU for different durations on the proliferation of the cell line HCT-116 is analyzed by the MTT assay. The morphological changes of HCT-116 cells are observed by the inverted photo microscopy after treatment with different concentrations of PB3A for 24 h. The 24 h apoptotic rates of the HCT-116 cells induced with PB3A and 5-FU are determined by the FCM with Annexin V-FITC/PI double labeling. The results show that the PB3A has a significant inhibitory effect on the HCT-116 cell proliferation and in a time-and dose-dependent manner, and an additive or synergistic effect is shown with the common chemotherapy drug 5-FU. The PB3A could induce the typical changes of cell morphology in a dose-dependent manner. The FCM analysis shows that the PB3A could significantly increase the apoptosis rate in a dosedependent manner. The findings in this study suggest that the PB3A can play a potential anticancer role for the colon cancer in vitro, and it could induce the apoptosis.
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