研究论文

西芹腐根浸提液处理后黄瓜枯萎病菌致弱机制

  • 李杰 ,
  • 云兴福 ,
  • 高晓敏 ,
  • 王勇 ,
  • 钱程 ,
  • 马立国
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  • 内蒙古农业大学农学院, 呼和浩特 010019
李杰,博士研究生,研究方向为西芹浸提液对黄瓜枯萎病菌的化感作用,电子信箱:lmljt0106@sina.cn

收稿日期: 2013-12-23

  修回日期: 2014-04-14

  网络出版日期: 2014-05-29

基金资助

国家自然科学基金项目(31160100);内蒙古自治区应用技术研究与开发项目(20110711)

Attenuation Mechanism of Parsley Rotten Root Extract on Fusarium oxysporum f. sp. cucumerinum

  • LI Jie ,
  • YUN Xingfu ,
  • GAO Xiaomin ,
  • WANG Yong ,
  • QIAN Cheng ,
  • MA Liguo
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  • College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China

Received date: 2013-12-23

  Revised date: 2014-04-14

  Online published: 2014-05-29

摘要

室内条件下用西芹腐根乙醇、丙酮和蒸馏水浸提液,连续5 代处理黄瓜枯萎病菌,测定处理后黄瓜枯萎病菌分泌的镰刀菌酸量和细胞壁降解酶活性及其与致病力的相关关系,以揭示西芹腐根浸提液连续多代处理黄瓜枯萎病菌的致弱机制。结果表明,各处理的致病力随接种代数的增加逐渐减小,镰刀菌酸质量浓度和果胶酶活性随培养代数的增加逐代减少,纤维素酶活性随培养代数的增加无明显的变化规律,β-葡萄糖苷酶活性随培养代数的增加逐代升高,但均表现为处理低于对照。相关分析表明,致病力与菌体分泌的镰刀菌酸质量浓度和果胶酶活性均表现为显著或极显著的正相关关系,与纤维素酶活性相关性不显著;与培养第3~5 代β-葡萄糖苷酶活性表现为显著或极显著的正相关关系。

本文引用格式

李杰 , 云兴福 , 高晓敏 , 王勇 , 钱程 , 马立国 . 西芹腐根浸提液处理后黄瓜枯萎病菌致弱机制[J]. 科技导报, 2014 , 32(14) : 24 -30 . DOI: 10.3981/j.issn.1000-7857.2014.14.003

Abstract

This paper investigates the attenuation mechanism of parsley rotten root extract on Fusarium oxysporum f. sp. cucumerinum (Foc.). Foc. was treated from the first to fifth generation with parsley rotten root extract prepared by ethanol, acetone, and distilled water. The excretive fusarium acid content and cell wall degradation enzyme activity were detected and their correlation with pathogenicity was studied. The results show that the pathogen's fusarium acid content and pectinase enzyme activity were significantly reduced with the increase of treated generations, the cellulose enzyme activity had no apparent change with the increase of treated generations, and the β-glycosidase enzyme activity increased with the increase of treated generations, but all were lower than those in the control group. Correlation analysis shows that there are significant or extremely significant positive correlation between pathogenicity and the pathogen's fusarium acid content, pectinase enzyme activity, and β-glycosidase enzyme activity, but there is no clear relationship between pathogenicity and the cellulose enzyme activity.

参考文献

[1] 张国华. 盆栽下西芹根物质对黄瓜枯萎病菌的化感作用及其对相关酶的影响[D]. 呼和浩特: 内蒙古农业大学, 2008. Zhang Guohua. Allelopathy of extract and rhizosphere in root of west celery on Fusarium oxysporium f. sp. cucumerinum and the effect of related enzymes in fotted condition[D]. Hohhot: Inner Mongolia Agricultural University, 2008.
[2] 林冬枝, 董彦君, 续荣治. 麦冬块根提取物对稻瘟病菌丝及苗期稻瘟病发生的抑制效果[J]. 中国农学通报, 2009, 25(9): 220-223. Lin Dongzhi, Dong Yanjun, Xu Rongzhi. Inhibitory effects of extracts from dwarf lilyturf (Ophiopogon japonicus K.) roots on rice blast fungus and rice blast disease at seedling stage[J]. Chinese Agricultural Science Bulletin, 2009, 25(9): 220-223.
[3] 贾俊英. 西芹种子浸提液对黄瓜枯萎病菌的化感作用[J]. 生态学杂 志, 2011, 30(7): 1473-1478. Jia Junying. Allelopathy of parsley seed extracts on Fusarium oxysporium f. sp. cucumerinum[J]. Chinese Journal of Ecology, 2011, 30 (7): 1473-1478.
[4] Da Silva J A, Pegado C M A, Ribeiro V V, et al. Effect of naturals extracts on the control of Fusarium oxysporum f. sp. tracheiphilum in cowpea seeds[J]. Cienciae Agrotecnologia, 2009, 33: 611-616.
[5] 曹阳. 西芹腐根及根际区物浸提液对黄瓜枯萎病菌化感作用的研究[D]. 呼和浩特: 内蒙古农业大学, 2009. Cao Yang. The study on the allelopathy of extracts of the rotten root and the rhizosphere zone rot soil of parsley on Fusarium oxysporum f. sp. cucumerinum[D]. Hohhot: Inner Mongolia Agricultural University, 2009.
[6] 贾俊英. 西芹种子浸提液对黄瓜枯萎病菌化感作用及其机理的研究[D]. 呼和浩特: 内蒙古农业大学, 2011. Jia Junying. Studies on allelopathy and mechanism of the extract solution of the parsley seed on Fusarium oxysporium f. sp. cucumerinum[D]. Hohhot: Inner Mongolia Agricultural University, 2011.
[7] 包妍妍. 西芹根际区物浸提液处理后黄瓜叶片内酚类物质、氮代谢物 质含量的变化[J]. 科技导报, 2013, 31(4): 60-66. Bao Yanyan. Changes of phenol and nitrogen metabolites contents in cucumber leaves treated with the extracts of celery root soil[J]. Science & Technology Review, 2013, 31(4): 60-66.
[8] 钱程, 高晓敏, 包妍妍, 等. 西芹种子浸提液处理后黄瓜枯萎病菌弱毒 菌株的筛选[J]. 生态学杂志, 2013, 32(1): 82-90. Qian Cheng, Gao Xiaomin, Bao Yanyan, et al. Screening of attenuated strains for Fusarium oxysporum f. sp. cucumerinum after treated with parsley seed extracts[J]. Chinese Journal of Ecology, 2013, 32(1): 82-90.
[9] 钱程, 云兴福, 高晓敏, 等. 西芹鲜根浸提液作用后黄瓜枯萎病菌弱毒 菌株的筛选[J]. 中国生态农业学报, 2013, 21(5): 606-614. Qian Cheng, Yun Xingfu, Gao Xiaomin, et al. Screening of virulenceattenuated strains for Fusarium oxysporum f. sp. cucumerinum after treatment with parsley fresh root extract[J]. Chinese Journal of Eco-Agriculture, 2013, 21(5): 606-614.
[10] 钱程. 西芹浸提液处理黄瓜枯萎病菌弱毒菌株的筛选及其诱导抗性 的研究[D]. 呼和浩特: 内蒙古农业大学, 2013. Qian Cheng. Study on the screening of attenuated strains for Fusarium oxysporum f. sp. cucumerinum and induced resistance on cucumber wilt after treated with parsley extracts from fresh root, rhizosphere soil and seed[D]. Hohhot: Inner Mongolia Agricultural University, 2013.
[11] Marcus L, Barash I, Sneh B, et al. Purification and characterization of pectolytic enzymes produced by virulent and hypovirulent isolates of Rhizoctonia solanikuhn[J]. Physiological and Molecular Plant Pathology, 1986, 29: 325-326.
[12] Mandels M, Hontz L, Nystrom J, et al. Enzymatic hydrolysis of waste cellulose[J]. Biotechnology and Bioengineering, 1974, 16: 1471-1493.
[13] 周红梅, 毛爱军, 张丽蓉, 等. 黄瓜枯萎病接种方法及抗性遗传的研 究[J]. 华北农学报, 2010, 25(4): 186-190. Zhou Hongmei, Mao Aijun, Zhang Lirong, et al. Research on inoculation method and the inheritance of resistance to Fusarium oxysporum f. sp. cucumerinum on cucumber[J]. Acta Agriculturae Boreali-Sinica, 2010, 25(4): 186-190.
[14] 王纯利, 王冬梅, 黄炜, 等. 西瓜枯萎病菌致病力与镰刀菌酸和β -1,4 葡萄糖苷酶活性的关系研究[J]. 新疆农业大学学报, 2000, 23(1): 1-6. Wang Chunli, Wang Dongmei, Huang Wei, et al. Study on the relationship between the fusaric acid, β-Glucoseidase activity and the pathogenicity of Fusarium oxysporum f. sp. Niveumon watermelon[J]. Journal of Xinjiang Agricultural Universit, 2000, 23(1): 1-6.
[15] 董汉松, 王智发. 烟草赤星病菌致病力分化与弱毒株抗性诱导作用 的研究[J]. 植物保护学报, 1992, 19(1): 87-90. Dong Hansong, Wang Zhifa. Pathogenicity differentiation of altern ariaalternata and induced resistance of tobacco to brown spot by weak virulence strains[J]. Acta Phytophylacica Sinica, 1992, 19(1): 87-90.
[16] 李忠福, 徐建国. 分光光度法测定果胶酶活性方法的研究[J]. 黑龙江 医药, 2002, 15(6): 428-431. Li Zhongfu, Xu Jianguo. Determination of pectinase activity by spectrophotometry[J]. Heilongjiang Medical Journal, 2002, 15(6): 428-431.
[17] 石延霞, 李宝聚. 黄瓜霜霉病菌致病作用与两种细胞壁降解酶关系 初探[J]. 园艺学报, 2003, 30(4): 465-466. Shi Yanxia, Li Baoju. The relation of pathogenesis action by Pseudoperonospora cubensis and two sort of zymin in cucumber[J]. Acta Horticulturae Sinica, 2003, 30(4): 465-466.
[18] 蒋荷, 曹莎, 王丽君, 等. 黄瓜枯萎病研究进展及其综合防治[J]. 中国 植保导刊, 2012, 32(11): 13-17. Jiang He, Cao Sha, Wang Lijun, et al. Research progress on cucumber Fusarium wilt and its integrated control[J]. Chian Plant Protection, 2012, 32(11): 13-17.
[19] 曹永军, 程萍, 喻国辉, 等. 香蕉枯萎病菌菌株致病力分化及其原因 研究[J]. 热带作物学报, 2011, 32(8): 1532-1536. Cao Yongjun, Cheng Ping, Yu Guohui, et al. Pathogenic differentiation of different Fusarium oxysporum f. sp. cubense strains[J]. Chinese Journal of Tropical Crops, 2011, 32(8): 1532-1536.
[20] 周凯南, 张立修, 吕士恩, 等. 西瓜枯萎病菌致病力的测定[J]. 山东农业大学学报, 1990(1): 43-46. Zhou Kainan, Zhang Lixiu, Lü Shien, et al. The determination of watermelon wilt pathogen pathogenicity[J]. Journal of Shandong Agricultural University, 1990(1): 43-46.
[21] 董章勇, 王振中. 植物病原真菌细胞壁降解酶的研究进展[J]. 湖北农 业学报, 2012, 51(21): 4697-4700. Dong Zhangyong, Wang Zhenzhong. Research progress of fungal cell wall-degrading enzyme[J]. Hubei Agricultural Sciences, 2012, 51(21): 4697-4700.
[22] 陈夕军, 张红, 徐敬友, 等. 水稻纹枯病胞壁降解酶的产生及致病作 用[J]. 江苏农业学报, 2006, 22(2): 24-28. Chen Xijun, Zhang Hong, Xu Jingyou, et al. Cell wall degrading enzymes produced by Rhizoctonia solani and their pathogenicity to rice plants[J]. Jiangsu Journal of Agricultural Sciences, 2006, 22(1): 24-28.
[23] Cano-Canchola C, Acevevdol, Ponce-Noyolap, et al. Induction of lytic by the interaction of ustilago witIlzea mays tissues[J]. Fungal Genetics and Biology, 2000, 29(3): 145-151.
[24] Li B J, Peng X W, Liu X L, et al. Study on attenuation mechanism of an avirulent strain in a melon[J]. Jagric Biotech, 2000, 10(S3): 85-87.
[25] 钱凌玉. 四川省核盘菌对多菌灵抗药性检测及致病机理研究[D]. 雅安: 四川农业大学, 2009. Qian Lingyu. Studies on resistanee to carbendazim and pathogeneity of Selerotinia selerotirum in Sichuan[D]. Yaan: Siehuan Agricultural University, 2009.
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